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Part:BBa_K1588004:Design

Designed by: Juan David Sifontes   Group: iGEM15_UNA_Honduras   (2015-09-18)


tetravalen protein with epitopes from dengue virus to generate immune response


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2020
    Illegal PstI site found at 2173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2020
    Illegal PstI site found at 2173
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2020
    Illegal PstI site found at 2173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2020
    Illegal PstI site found at 2173
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706
    Illegal SapI.rc site found at 1694


Design Notes

1. is only one polypeptide chain 2. bacteria cells export them 3. low molecular weight 4. big exposed loops where we could insert antigenic determinants (epitopes)



Source

from Vibrio cholerae gene for Chitin Oligosaccharide Binding Protein

References